Novel Mediation Analysis of Human Plasma Proteome and Metabolome Reveals Mediators of Improved Glycemia After Gastric Bypass Surgery
Jonathan Dreyfuss PhD; Instructor in Medicine, Harvard Medical School; Associate Director of Bioinformatics and Biostatistics Core, Joslin Diabetes Center
Molecular mechanisms by which Roux-en-Y gastric bypass (RYGB) improves glycemic control and metabolism in type 2 diabetes (T2D) remain incompletely understood. In the SLIMM-T2D trial, participants with T2D were randomized to RYGB or nonsurgical management and their fasting plasma proteome and metabolome were analyzed for up to three years. To identify analytes that mediate improvement in outcomes, we developed a high-throughput mediation analysis method (Hitman), which is significantly more powerful than existing methods.